Lysozyme-lysozyme self-interactions as assessed by the osmotic second virial coefficient: Impact for physical protein stabilization
نویسندگان
چکیده
منابع مشابه
Second virial coefficient studies of cosolvent-induced protein self-interaction.
Protein self-interaction is important in protein crystal growth, solubilization, and aggregation, both in vitro and in vivo, as with protein misfolding diseases, such as Alzheimer's. Although second virial coefficient studies can supply invaluable quantitative information, their emergence as a systematic approach to evaluating protein self-interaction has been slowed by the limitations of tradi...
متن کاملWhy is the osmotic second virial coefficient related to protein crystallization?
A molecular basis is presented for characterizing the osmotic second virial coefficient, B 22 , of dilute protein solutions, which provides a measure of the nature of protein—protein interactions and has been shown to be correlated with crystallization behavior. Experimental measurements of the second virial coefficient of lysozyme and bovine a-chymotrypsinogen A were performed by static light ...
متن کاملCalculation of weak protein-protein interactions: the pH dependence of the second virial coefficient.
Interactions between proteins are often sufficiently weak that their study through the use of conventional structural techniques becomes problematic. Of the few techniques capable of providing experimental measures of weak protein-protein interactions, perhaps the most useful is the second virial coefficient, B(22), which quantifies a protein solution's deviations from ideal behavior. It has lo...
متن کاملMolecular Weight, Osmotic Second Virial Coefficient, and Extinction Coefficient of Colloidal CdSe Nanocrystals
Membrane osmometry is used to measure osmotic pressures of dilute solutions containing quasi-spherical CdSe nanocrystals covered with polymer brushes in toluene in the range 31 to 45°C. Osmotic-pressure data, as a function of nanocrystal concentration, yield the molecular weight and the osmotic second virial coefficient of the nanocrystals; the latter is related to the potential of mean force b...
متن کاملApplications of the second virial coefficient: protein crystallization and solubility
A number of citations in the article by Wilson & DeLucas [(2014). Acta Cryst. F70, 543-554] are corrected.
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ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2009
ISSN: 1860-6768
DOI: 10.1002/biot.200800274